Cat CRP(C Reactive Protein) ELISA Kit

Cat CRP(C Reactive Protein) ELISA Kit



SKU: ELK8881 Category:

Overview Of Cat CRP(C Reactive Protein) ELISA Kit

Product name: Human NFκB-p65(Nuclear Factor Kappa B p65) ELISA Kit
Reactivity: Human
Alternative Names: NFKB3; p65; RelA
Assay Type: Sandwich
Sensitivity: 0.10 ng/mL
Standard: 10 ng/mL
Detection Range: 0.16-10 ng/mL
Sample Type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length: 3.5h
Research Area: Signal transduction;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human NFκB-p65. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human NFκB-p65. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human NFκB-p65, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human NFκB-p65 in the samples is then determined by comparing the OD of the samples to the standard curve.


Additional information


48R, 96R


There are no reviews yet.

Be the first to review “Cat CRP(C Reactive Protein) ELISA Kit”

Your email address will not be published. Required fields are marked *