Cat CRP(C Reactive Protein) ELISA Kit
Overview Of Cat CRP(C Reactive Protein) ELISA Kit
Product name: | Human NFκB-p65(Nuclear Factor Kappa B p65) ELISA Kit |
Reactivity: | Human |
Alternative Names: | NFKB3; p65; RelA |
Assay Type: | Sandwich |
Sensitivity: | 0.10 ng/mL |
Standard: | 10 ng/mL |
Detection Range: | 0.16-10 ng/mL |
Sample Type: | serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids |
Assay Length: | 3.5h |
Research Area: | Signal transduction; |
Test principle: | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human NFκB-p65. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human NFκB-p65. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human NFκB-p65, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human NFκB-p65 in the samples is then determined by comparing the OD of the samples to the standard curve. |
Description
Additional information
Size | 48R, 96R |
---|
Reviews
There are no reviews yet.