Human NT-ProBNP(N-Terminal Pro-Brain Natriuretic Peptide) ELISA Kit

Human NT-ProBNP(N-Terminal Pro-Brain Natriuretic Peptide) ELISA Kit

364.00

Specification 96T, 48T

SKU: ELK1212 Category: Tag:

Overview of Human NT-ProBNP(N-Terminal Pro-Brain Natriuretic Peptide) ELISA Kit

Product name: Human NT-ProBNP(N-Terminal Pro-Brain Natriuretic Peptide) ELISA Kit
Reactivity: Human
Alternative Names: NT-Pro-BNP; ; N-BNP; ANFB_HUMAN; BNP(1-32); BNP(5-29); BNP-32; Gamma-brain natriuretic peptide; nppb
Assay Type: Sandwich
Sensitivity: 15 pg/mL
Standard: 2500 pg/mL
Detection Range: 39.07-2500 pg/mL
Sample Type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length: 3.5h
Research Area: Endocrinology;Cardiovascular biology;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human NT-ProBNP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human NT-ProBNP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After the TMB substrate solution is added, only those wells that contain Human NT-ProBNP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a colour change. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human NT-ProBNP in the samples is then determined by comparing the OD of the samples to the standard curve.

 

Read More Description

Additional information

Size

48T, 96T, 96T,48T