Human TRF(Transferrin) ELISA Kit

Human TRF(Transferrin) ELISA Kit


Specification 96T, 48T

SKU: ELK1300 Category: Tag:

Overview of Human TRF(Transferrin) ELISA Kit

Product name: Human TRF(Transferrin) ELISA Kit
Reactivity: Human
Alternative Names: TF; Siderophilin; Serotransferrin; Beta-1 metal-binding globulin
Assay Type: Sandwich
Sensitivity: 0.68 ng/mL
Standard: 100 pg/mL
Detection Range: 1.57-100 pg/mL
Sample Type: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length: 3.5 h
Research Area: Tumor immunity;Infection immunity;Immune molecule;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human TRF. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human TRF. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After the TMB substrate solution is added, only those wells that contain Human TRF, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a colour change. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human TRF in the samples is then determined by comparing the OD of the samples to the standard curve.


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48T, 96T, 96T,48T

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