Mouse VEGFA(Vascular Endothelial Growth Factor A) ELISA Kit

Mouse VEGFA(Vascular Endothelial Growth Factor A) ELISA Kit

262.00

Specification 96T, 48T

SKU: ELK1293 Category: Tag:

Overview of Mouse VEGFA(Vascular Endothelial Growth Factor A) ELISA Kit

Product name: Mouse VEGFA(Vascular Endothelial Growth Factor A) ELISA Kit
Reactivity: Mouse
Alternative Names: VEGF; VEGF-A; VPF; Vascular Endothelial Cell Growth Factor A; Vascular Permeability Factor
Assay Type: Sandwich
Sensitivity: 5.7 pg/mL
Standard: 1000 pg/mL
Detection Range: 15.63-1000 pg/mL
Sample Type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length: 3.5 h
Research Area: Cytokine;Apoptosis;Tumor immunity;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse VEGFA. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse VEGFA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After the TMB substrate solution is added, only those wells that contain Mouse VEGFA, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a colour change. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse VEGFA in the samples is then determined by comparing the OD of the samples to the standard curve.

Read More Description

Additional information

Size

48T, 96T, 96T,48T

Top rated products