Rat GHRH-Growth Hormone Releasing Hormone ELISA Kit

Rat GHRH-Growth Hormone Releasing Hormone ELISA Kit

318.00455.00

Specification 96T, 48T

SKU: ELK2413 Category: Tag:

Overview of Rat GHRH-Growth Hormone Releasing Hormone ELISA Kit

Product name: Rat GHRH-Growth Hormone Releasing Hormone ELISA Kit
Reactivity: Rat
Alternative Names: GRF; GHRF; Somatocrinin; Growth-Hormone-Releasing Factor; Sermorelin; Somatoliberin
Assay Type: Competitive Inhibition
Sensitivity: 4.54 pg/mL
Standard: 1000 ng/mL
Detection Range: 15.63-1000 pg/mL
Sample Type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length: 2 h
Research Area: Endocrinology;Hormone metabolism;
Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Rat GHRH protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat GHRH. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat GHRH in the samples is then determined by comparing the OD of the samples to the standard curve.

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Size

48T, 96T, 96T,48T

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