Rat LEP(Leptin) ELISA Kit

Rat LEP(Leptin) ELISA Kit

262.00

Specification 96T, 48T

SKU: ELK1244 Category: Tag:

Overview of Rat LEP(Leptin) ELISA Kit

Product name: Rat LEP(Leptin) ELISA Kit
Reactivity: Rat
Alternative Names: OB; OBS; Obesity Homolog; Obesity Factor; Obese Protein
Assay Type: Sandwich
Sensitivity: 0.129 ng/mL
Standard: 20 ng/mL
Detection Range: 0.32-20 ng/mL
Sample Type: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length: 3.5h
Research Area: Metabolic pathway;Endocrinology;Cardiovascular biology;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat LEP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat LEP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After the TMB substrate solution is added, only those wells that contain Rat LEP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a colour change. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat LEP in the samples is then determined by comparing the OD of the samples to the standard curve.

 

Read More Description

Additional information

Size

48T, 96T, 96T,48T