Human FAS-Factor Related Apoptosis ELISA Kit
Overview of Human FAS-Factor Related Apoptosis ELISA Kit
Product name: | Human FAS-Factor Related Apoptosis ELISA Kit |
Reactivity: | Human |
Alternative Names: | CD95; ALPS1A; ALPS1-A; APO1; APT1; FAS1; FASTM; TNFRSF6; Fas Receptor; TNF Receptor Superfamily Member 6; Tumor Necrosis Factor Receptor Superfamily Member 6 |
Assay Type: | Sandwich |
Sensitivity: | 15 pg/mL |
Standard: | 2500 pg/mL |
Detection Range: | 39.07-2500 pg/mL |
Sample Type: | serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids |
Assay Length: | 3.5h |
Research Area: | Apoptosis;Tumor immunity; |
Test principle: | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human FAS. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human FAS. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human FAS, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human FAS in the samples is then determined by comparing the OD of the samples to the standard curve. |
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One Step SYBR Green RT-PCR Mix
Additional information
Size | 48T, 96T, 96T,48T |
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