Human GCSF-Colony Stimulating Factor 3, Granulocyte ELISA Kit
Human GCSF-Colony Stimulating Factor 3, Granulocyte ELISA Kit
€262.00 – €364.00
Specification 96T, 48T
Overview of Human GCSF-Colony Stimulating Factor 3, Granulocyte ELISA Kit
Product name: | Human GCSF-Colony Stimulating Factor 3, Granulocyte ELISA Kit |
Reactivity: | Human |
Alternative Names: | CSF3; G-CSF; Granulocyte Colony Stimulating Factor; Pluripoietin; Filgrastim; Lenograstim |
Assay Type: | Sandwich |
Sensitivity: | 5.5 pg/mL |
Standard: | 1000 pg/mL |
Detection Range: | 15.63-1000 pg/mL |
Sample Type: | serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids |
Assay Length: | 3.5h |
Research Area: | Cytokine;Hematology; |
Test principle: | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human GCSF. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human GCSF. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human GCSF, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human GCSF in the samples is then determined by comparing the OD of the samples to the standard curve. |
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Additional information
Size | 48T, 96T, 96T,48T |
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