Overview of Human GC-Glucagon ELISA Kit
Product name: |
Human GC-Glucagon ELISA Kit |
Reactivity: |
Human |
Alternative Names: |
GCG; GLP1; GLP2; GRPP; Glicentin-Related Polypeptide; Glucagen; Oxyntomodulin; Incretin hormone |
Assay Type: |
Competitive Inhibition |
Sensitivity: |
7.54 pg/mL |
Standard: |
1500 pg/mL |
Detection Range: |
23.44-1500 pg/mL |
Sample Type: |
serum, plasma and other biological fluids |
Assay Length: |
2 h |
Research Area: |
Endocrinology; |
Test principle: |
This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human GC protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human GC. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human GC in the samples is then determined by comparing the OD of the samples to the standard curve. |
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