Human S100A8-S100 Calcium Binding Protein A8 ELISA Kit
Human S100A8-S100 Calcium Binding Protein A8 ELISA Kit
€262.00 – €364.00
Specification 96T, 48T
Overview of Human S100A8-S100 Calcium Binding Protein A8 ELISA Kit
| Product name: | Human S100A8-S100 Calcium Binding Protein A8 ELISA Kit |
| Reactivity: | Human |
| Alternative Names: | MRP8; MIF; 60B8AG; CAGA; CFAG; CGLA; CP-10; L1Ag; NIF; P8; Calgranulin A; Cystic fibrosis antigen; Leukocyte L1 complex light chain; Migration inhibitory factor-related protein 8 |
| Assay Type: | Sandwich |
| Sensitivity: | 5.6 pg/mL |
| Standard: | 1000 pg/mL |
| Detection Range: | 15.63-1000 pg/mL |
| Sample Type: | serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids |
| Assay Length: | 3.5 h |
| Research Area: | Tumor immunity;Infection immunity; |
| Test principle: | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human S100A8. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human S100A8. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human S100A8, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human S100A8 in the samples is then determined by comparing the OD of the samples to the standard curve. |
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Additional information
| Size | 48T, 96T, 96T,48T |
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