Name: Human IGFBP3-Insulin Like Growth Factor Binding Protein 3 ELISA Kit - Gencyst
SKU: ELK1139
Availability: InStock

Human IGFBP3-Insulin Like Growth Factor Binding Protein 3 ELISA Kit

€262.00 – €364.00

Specification 96T, 48T

Size

Clear

SKU: ELK1139 Category: ELISA Kit Tag: ELISA Kit

Overview of Human IGFBP3-Insulin Like Growth Factor Binding Protein 3 ELISA Kit

Product name:	Human IGFBP3-Insulin Like Growth Factor Binding Protein 3 ELISA Kit
Reactivity:	Human
Alternative Names:	BP53; IBP3; Growth Hormone-Dependent Binding Protein; Acid Stable Subunit Of The 140 K IGF Complex
Assay Type:	Sandwich
Sensitivity:	0.071 ng/mL
Standard:	10 pg/mL
Detection Range:	0.16-10 ng/mL
Sample Type:	Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Assay Length:	3.5 h
Research Area:	Metabolic pathway;Tumor immunity;
Test principle:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human IGFBP3. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human IGFBP3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human IGFBP3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human IGFBP3 in the samples is then determined by comparing the OD of the samples to the standard curve.

Size	48T, 96T

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