Human IL4-Interleukin 4 ELISA Kit
Human IL4-Interleukin 4 ELISA Kit
€262.00 – €374.00
Specification 96T, 48T
Overview of Human IL4-Interleukin 4 ELISA Kit
| Product name: | Human IL4-Interleukin 4 ELISA Kit |
| Reactivity: | Human |
| Alternative Names: | IL-4;BSF1; BCGF1 ; B Cell Stimulatory Factor 1; Lymphocyte stimulatory factor 1; B Cell Growth Factor; Binetrakin; Pitrakinra |
| Assay Type: | Sandwich |
| Sensitivity: | 5.7 pg/mL |
| Standard: | 1000 pg/mL |
| Detection Range: | 15.63-1000 pg/mL |
| Sample Type: | serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids |
| Assay Length: | 3.5 h |
| Research Area: | Cytokine;Immune molecule; |
| Test principle: | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human IL4. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human IL4. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human IL4, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human IL4 in the samples is then determined by comparing the OD of the samples to the standard curve. |
Read More Description
One Step SYBR Green RT-PCR Mix
Additional information
| Size | 48T, 96T |
|---|
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