Human Tie1-Tyrosine Kinase With Immunoglobulin Like And EGF Like Domains Protein 1 ELISA Kit
Human Tie1-Tyrosine Kinase With Immunoglobulin Like And EGF Like Domains Protein 1 ELISA Kit
€262.00 – €374.00
Specification 96T, 48T
Overview of Human Tie1-Tyrosine Kinase With Immunoglobulin Like And EGF Like Domains Protein 1 ELISA Kit
Product name: | Human Tie1-Tyrosine Kinase With Immunoglobulin Like And EGF Like Domains Protein 1 ELISA Kit |
Reactivity: | Human |
Alternative Names: | JTK14; Tyrosine Kinase With Immunoglobulin And Epidermal Growth Factor Homology Domains 1; Tyrosine-protein kinase receptor Tie-1 |
Assay Type: | Sandwich |
Sensitivity: | 0.62 ng/mL |
Standard: | 100 pg/mL |
Detection Range: | 1.57-100 ng/mL |
Sample Type: | serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids |
Assay Length: | 3.5 h |
Research Area: | Tumor immunity;Infection immunity; |
Test principle: | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human Tie1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human Tie1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human Tie1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human Tie1 in the samples is then determined by comparing the OD of the samples to the standard curve. |
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One Step SYBR Green RT-PCR Mix
Additional information
Size | 48T, 96T |
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