Overview of Human FE-Ferritin ELISA Kit
Product name: |
Human FE-Ferritin ELISA Kit |
Reactivity: |
Human |
Alternative Names: |
Ferritin |
Assay Type: |
Sandwich |
Sensitivity: |
0.061 ng/mL |
Standard: |
10 ng/mL |
Detection Range: |
0.16-10 ng/mL |
Sample Type: |
plasma |
Assay Length: |
3.5 h |
Research Area: |
Metabolic pathway;Tumor immunity;Hematology; |
Test principle: |
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human FE. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human FE. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human FE, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human FE in the samples is then determined by comparing the OD of the samples to the standard curve. |
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