Rat IgM-Immunoglobulin M ELISA Kit

Rat IgM-Immunoglobulin M ELISA Kit

262.00364.00

Specification 96T, 48T

SKU: ELK1349 Category: Tag:

Overview of Rat IgM-Immunoglobulin M ELISA Kit

Product name: Rat IgM-Immunoglobulin M ELISA Kit
Reactivity: Rat
Alternative Names: IGHM; Immunoglobulin Heavy Constant Mu; Ig mu chain C region
Assay Type: Sandwich
Sensitivity: 0.069 ÎĽg/mL
Standard: 10 ng/mL
Detection Range: 0.16-10 ng/mL
Sample Type: serum and plasma
Assay Length: 3.5 h
Research Area: Infection immunity;Immune molecule;Hematology;
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat IgM. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat IgM. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat IgM, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat IgM in the samples is then determined by comparing the OD of the samples to the standard curve.

Read More Description

dNTP Mix PCR Grade 10 mM each

Pfu DNA Polymerase

One Step SYBR Green RT-PCR Mix

Hot start Taq DNA Polymerase

EntiLink PCR Master Mix

Additional information

Size

48T, 96T, 96T,48T

Reviews

There are no reviews yet.

Be the first to review “Rat IgM-Immunoglobulin M ELISA Kit”

Your email address will not be published. Required fields are marked *

Filter by price