Name: Rat IgG-Immunoglobulin G ELISA Kit - Gencyst
SKU: ELK1393
Availability: InStock

Rat IgG-Immunoglobulin G ELISA Kit

€262.00 – €364.00

Specification 96T, 48T

SKU: ELK1393 Category: ELISA Kit Tag: ELISA Kit

Overview of Rat IgG-Immunoglobulin G ELISA Kit

Product name:	Rat IgG-Immunoglobulin G ELISA Kit
Reactivity:	Rat
Alternative Names:	Immunoglobulin G
Assay Type:	Sandwich
Sensitivity:	1.88 μg/mL
Standard:	200 ng/mL
Detection Range:	3.13-200 μg/mL
Sample Type:	serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length:	3.5 h
Research Area:	Infection immunity;Immune molecule;Hematology;
Test principle:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat IgG. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat IgG. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat IgG, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat IgG in the samples is then determined by comparing the OD of the samples to the standard curve.

Size	48T, 96T

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