Overview of Human HMGB-1-High Mobility Group Protein B1 ELISA Kit
Product name: |
Human HMGB-1-High Mobility Group Protein B1 ELISA Kit |
Reactivity: |
Human |
Alternative Names: |
HMGB1; HMG3; SBP1; Sulfoglucuronyl Carbohydrate Binding Protein; Amphoterin; High Mobility Group Box 1 Protein; HMG1; High Mobility Group Protein 1 |
Assay Type: |
Sandwich |
Sensitivity: |
28.3 pg/mL |
Standard: |
4000 ng/mL |
Detection Range: |
62.5-4000 pg/mL |
Sample Type: |
serum, plasma and other biological fluids |
Assay Length: |
3.5 h |
Research Area: |
Infection immunity; |
|
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human HMGB-1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human HMGB-1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human HMGB-1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human HMGB-1 in the samples is then determined by comparing the OD of the samples to the standard curve. |
dNTP Mix PCR Grade 10 mM each
Pfu DNA Polymerase
One Step SYBR Green RT-PCR Mix
Hot start Taq DNA Polymerase
EntiLink PCR Master Mix
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