Name: Rat aFP-Alpha-Fetoprotein ELISA Kit - Gencyst
SKU: ELK1482
Availability: InStock

Rat aFP-Alpha-Fetoprotein ELISA Kit

€318.00 – €455.00

Specification 96T, 48T

SKU: ELK1482 Category: ELISA Kit Tag: ELISA Kit

Overview of Rat aFP-Alpha-Fetoprotein ELISA Kit

Product name:	Rat aFP-Alpha-Fetoprotein ELISA Kit
Reactivity:	Rat
Alternative Names:	A-FP; FETA; HPAFP; Alpha-Fetoglobulin; Alpha-1-fetoprotein
Assay Type:	Sandwich
Sensitivity:	0.127 ng/mL
Standard:	20 ng/mL
Detection Range:	0.32-20 ng/mL
Sample Type:	serum, plasma, tissue homogenates and other biological fluids
Assay Length:	3.5 h
Research Area:	Tumor immunity;Hepatology;
	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat aFP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat aFP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat aFP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat aFP in the samples is then determined by comparing the OD of the samples to the standard curve.

Size	48T, 96T

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