Name: Human TP53-Tumor Protein p53 ELISA Kit - Gencyst
SKU: ELK1507
Availability: InStock

Human TP53-Tumor Protein p53 ELISA Kit

€318.00 – €455.00

Specification 96T, 48T

SKU: ELK1507 Category: ELISA Kit Tag: ELISA Kit

Overview of Human TP53-Tumor Protein p53 ELISA Kit

Product name:	Human TP53-Tumor Protein p53 ELISA Kit
Reactivity:	Human
Alternative Names:	p53; LFS1; TRP53; Li-Fraumeni Syndrome; Cellular tumor antigen p53; Antigen NY-CO-13; Phosphoprotein p53; Tumor suppressor p53
Assay Type:	Sandwich
Sensitivity:	27 pg/mL
Standard:	5000 ng/mL
Detection Range:	78.13-5000 pg/mL
Sample Type:	serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length:	3.5 h
Research Area:	Signal transduction;Metabolic pathway;Tumor immunity;
Test principle:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human TP53. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human TP53. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human TP53, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human TP53 in the samples is then determined by comparing the OD of the samples to the standard curve.

Size	48T, 96T

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