Rat F2-Coagulation Factor II ELISA Kit

Rat F2-Coagulation Factor II ELISA Kit

318.00455.00

Specification 96T, 48T

SKU: ELK1517 Category: Tag:

Overview of Rat F2-Coagulation Factor II ELISA Kit

Product name: Rat F2-Coagulation Factor II ELISA Kit
Reactivity: Rat
Alternative Names: FII; TM; PT; Thrombin; Prothrombin; Pro-Thrombin; Activation peptide fragment 1; Activation peptide fragment 2
Assay Type: Sandwich
Sensitivity: 3.5 ng/mL
Standard: 500 ng/mL
Detection Range: 7.82-500 ng/mL
Sample Type: Serum
Assay Length: 3.5 h
Research Area: Signal transduction;Hematology;
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat F2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat F2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat F2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The addition of sulphuric acid solution terminates the enzyme-substrate reaction and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat F2 in the samples is then determined by comparing the OD of the samples to the standard curve.

Read More Description

dNTP Mix PCR Grade 10 mM each

Pfu DNA Polymerase

One Step SYBR Green RT-PCR Mix

Hot start Taq DNA Polymerase

EntiLink PCR Master Mix

Additional information

Size

48T, 96T

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