Name: Rat PYGM-Glycogen Phosphorylase, Muscle ELISA Kit - Gencyst
SKU: ELK1576
Availability: InStock

Rat PYGM-Glycogen Phosphorylase, Muscle ELISA Kit

€318.00 – €455.00

Specification 96T, 48T

Size

Clear

SKU: ELK1576 Category: ELISA Kit Tag: ELISA Kit

Overview of Rat PYGM-Glycogen Phosphorylase, Muscle ELISA Kit

Product name:	Rat PYGM-Glycogen Phosphorylase, Muscle ELISA Kit
Reactivity:	Rat
Alternative Names:	GPMM; Phosphorylase,Glycogen; McArdle Syndrome,Glycogen Storage Disease Type V; Myophosphorylase
Assay Type:	Sandwich
Sensitivity:	0.054 ng/mL
Standard:	10 ng/mL
Detection Range:	0.16-10 ng/mL
Sample Type:	tissue homogenates, cell lysates and other biological fluids
Assay Length:	3.5 h
Research Area:	Enzyme & Kinase;Metabolic pathway;Endocrinology;
Test Principle:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat PYGM. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat PYGM. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat PYGM, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat PYGM in the samples is then determined by comparing the OD of the samples to the standard curve.

Size	48T, 96T

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