Overview of Mouse GIP-Gastric Inhibitory Polypeptide ELISA Kit
Product name: |
Mouse GIP-Gastric Inhibitory Polypeptide ELISA Kit |
Reactivity: |
Mouse |
Alternative Names: |
Incretin hormone;Â Glucose Dependent Insulinotropic Peptide |
Assay Type: |
Competitive Inhibition |
Sensitivity: |
25.2 pg/mL |
Standard: |
5000 ng/mL |
Detection Range: |
78.13-5000 pg/mL |
Sample Type: |
Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids |
Assay Length: |
2 h |
Research Area: |
Endocrinology;Gastroenterology;Hormone metabolism; |
Test Principle: |
This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Mouse GIP protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse GIP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse GIP in the samples is then determined by comparing the OD of the samples to the standard curve. |
dNTP Mix PCR Grade 10 mM each
Pfu DNA Polymerase
One Step SYBR Green RT-PCR Mix
Hot start Taq DNA Polymerase
EntiLink PCR Master Mix
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