Overview of Human LAB7-2-B-Lymphocyte Activation Antigen B7-2 ELISA Kit
Product name: |
Human LAB7-2-B-Lymphocyte Activation Antigen B7-2 ELISA Kit |
Reactivity: |
Human |
Alternative Names: |
CD86; B7-2; B70; FUN-1; CD28LG2; CD28 Antigen Ligand 2,B7-2 Antigen; CTLA-4 counter-receptor B7.2 |
Assay Type: |
Sandwich |
Sensitivity: |
0.274 ng/mL |
Standard: |
10 ng/mL |
Detection Range: |
0.16-10 ng/mL |
Sample Type: |
Tissue homogenates, cell lysates, cell culture supernates and other biological fluids |
Assay Length: |
3.5 h |
Research Area: |
Signal transduction;CD & Adhesion molecule;Tumor immunity;Infection immunity;Immune molecule; |
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The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human LAB7-2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human LAB7-2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human LAB7-2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human LAB7-2 in the samples is then determined by comparing the OD of the samples to the standard curve. |
dNTP Mix PCR Grade 10 mM each
Pfu DNA Polymerase
One Step SYBR Green RT-PCR Mix
Hot start Taq DNA Polymerase
EntiLink PCR Master Mix
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