Human PPAR-γ-Peroxisome Proliferator Activated Receptor Gamma ELISA Kit
Human PPAR-γ-Peroxisome Proliferator Activated Receptor Gamma ELISA Kit
€318.00 – €455.00
Specification 96T, 48T
Overview of Human PPAR-γ-Peroxisome Proliferator Activated Receptor Gamma ELISA Kit
Product name: | Human PPAR-γ-Peroxisome Proliferator Activated Receptor Gamma ELISA Kit |
Reactivity: | Human |
Alternative Names: | PPAR-G; PPARG1; PPARG2; NR1C3; Glitazone Receptor; Nuclear Receptor Subfamily 1 Group C Member 3; PPARg; Peroxisome Proliferator Activated Receptor Gamma |
Assay Type: | Sandwich |
Sensitivity: | 0.057 ng/mL |
Standard: | 10 ng/mL |
Detection Range: | 0.16-10 ng/mL |
Sample Type: | serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids |
Assay Length: | 3.5 h |
Research Area: | Metabolic pathway;Endocrinology;Cardiovascular biology;Developmental science;Bone metabolism; |
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human PPAR-γ. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human PPAR-γ. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human PPAR-γ, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human PPAR-γ in the samples is then determined by comparing the OD of the samples to the standard curve. |
Read More Description
Additional information
Size | 48T, 96T, 96T,48T |
---|
Reviews
There are no reviews yet.