Name: Human PKCe-Protein Kinase C Epsilon ELISA Kit - Gencyst
SKU: ELK1647
Availability: InStock

Human PKCe-Protein Kinase C Epsilon ELISA Kit

€318.00 – €455.00

Specification 96T, 48T

Size

Clear

SKU: ELK1647 Category: ELISA Kit Tag: ELISA Kit

Overview of Human PKCe-Protein Kinase C Epsilon ELISA Kit

Product name:	Human PKCe-Protein Kinase C Epsilon ELISA Kit
Reactivity:	Human
Alternative Names:	PKC-E; PRKCE; PRKC-E; NPKC-Epsilon
Assay Type:	Sandwich
Sensitivity:	0.54 ng/mL
Standard:	100 ng/mL
Detection Range:	1.57-100 ng/mL
Sample Type:	tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length:	3.5 h
Research Area:	Signal transduction;Enzyme & Kinase;Apoptosis;Endocrinology;Cardiovascular biology;
	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human PKCe. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human PKCe. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human PKCe, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human PKCe in the samples is then determined by comparing the OD of the samples to the standard curve.

Size	48T, 96T

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