Rat C4-Complement Component 4 ELISA Kit
Overview of Rat C4-Complement Component 4 ELISA Kit
Product name: |
Rat C4-Complement Component 4 ELISA Kit |
Reactivity: | Rat |
Alternative Names: | C4BP-a; Complement Component 4 Binding Protein,Alpha |
Assay Type: | Sandwich |
Sensitivity: | 2.74 ng/mL |
Standard: | 400 ng/mL |
Detection Range: | 6.25-400 ng/mL |
Sample Type: | Serum, plasma and other biological fluids |
Assay Length: | 3.5 h |
Research Area: | Signal transduction;Infection immunity;Hematology; |
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat C4. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat C4. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat C4, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat C4 in the samples is then determined by comparing the OD of the samples to the standard curve. |
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Additional information
Size | 48T, 96T |
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