Name: Mouse COL4-Collagen Type IV ELISA Kit - Gencyst
SKU: ELK1665
Availability: InStock

Mouse COL4-Collagen Type IV ELISA Kit

€318.00 – €455.00

Specification 96T, 48T

SKU: ELK1665 Category: ELISA Kit Tag: ELISA Kit

Overview of Mouse COL4-Collagen Type IV ELISA Kit

Product name:	Mouse COL4-Collagen Type IV ELISA Kit
Reactivity:	Mouse
Alternative Names:	Type-IV Collagen
Assay Type:	Sandwich
Sensitivity:	0.064 ng/mL
Standard:	10ng/mL
Detection Range:	0.16-10 ng/mL
Sample Type:	serum, plasma and other biological fluids
Assay Length:	3.5 h
Research Area:	Tumor immunity;Hepatology;
	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse COL4. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse COL4. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse COL4, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse COL4 in the samples is then determined by comparing the OD of the samples to the standard curve.

Size	48T, 96T

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