Overview of Human VTN-Vitronectin ELISA Kit
Product name: |
Human VTN-Vitronectin ELISA Kit |
Reactivity: |
Human |
Alternative Names: |
V75; VN; VNT; Serum Spreading Factor; Somatomedin B; Complement S-Protein |
Assay Type: |
Sandwich |
Sensitivity: |
2.74 ng/mL |
Standard: |
400 ng/mL |
Detection Range: |
6.25-400 ng/mL |
Sample Type: |
serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids |
Assay Length: |
3.5 h |
Research Area: |
Signal transduction;Metabolic pathway;Tumor immunity;Hepatology; |
Test Principle: |
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human VTN. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human VTN. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human VTN, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human VTN in the samples is then determined by comparing the OD of the samples to the standard curve. |
dNTP Mix PCR Grade 10 mM each
Pfu DNA Polymerase
One Step SYBR Green RT-PCR Mix
Hot start Taq DNA Polymerase
EntiLink PCR Master Mix
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