Overview of Human SPB-Surfactant Associated Protein B ELISA Kit
Product name: |
Human SPB-Surfactant Associated Protein B ELISA Kit |
Reactivity: |
Human |
Alternative Names: |
SFTPB; PSPB; SFTB3; SFTP3; SP-B; Pulmonary Surfactant Protein B; 18 kDa pulmonary-surfactant protein; 6 kDa protein |
Assay Type: |
Sandwich |
Sensitivity: |
0.61 ng/mL |
Standard: |
100 ng/mL |
Detection Range: |
1.57-100 ng/mL |
Sample Type: |
serum, plasma, lung lavage fluid, tissue homogenates, cell lysates, cell culture supernates and other biological fluids |
Assay Length: |
3.5 h |
Research Area: |
Tumor immunity;Infection immunity;Pulmonology; |
Test Principle: |
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human SPB. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human SPB. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human SPB, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human SPB in the samples is then determined by comparing the OD of the samples to the standard curve. |
dNTP Mix PCR Grade 10 mM each
Pfu DNA Polymerase
One Step SYBR Green RT-PCR Mix
Hot start Taq DNA Polymerase
EntiLink PCR Master Mix
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