Name: Human PRF1-Perforin 1 ELISA Kit - Gencyst
SKU: ELK1879
Availability: InStock

Human PRF1-Perforin 1 ELISA Kit

€318.00 – €455.00

Specification 96T, 48T

SKU: ELK1879 Category: ELISA Kit Tag: ELISA Kit

Overview of Human PRF1-Perforin 1 ELISA Kit

Product name:	Human PRF1-Perforin 1 ELISA Kit
Reactivity:	Human
Alternative Names:	FLH2; HPLH2; P1; PFP; Pore Forming Protein; Cytolysin; Lymphocyte pore-forming protein
Assay Type:	Sandwich
Sensitivity:	0.62 ng/mL
Standard:	100 pg/mL
Detection Range:	1.57-100 ng/mL
Sample Type:	Serum, plasma, tissue homogenates and other biological fluids
Assay Length:	3.5 h
Research Area:	Immune molecule;
Test Principle:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human PRF1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human PRF1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human PRF1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human PRF1 in the samples is then determined by comparing the OD of the samples to the standard curve.

Size	48T, 96T, 96T,48T

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