Human HCII-Heparin Cofactor II ELISA Kit
Overview of Human HCII-Heparin Cofactor II ELISA Kit
Product name: |
Human HCII-Heparin Cofactor II ELISA Kit |
Reactivity: | Human |
Alternative Names: | SERPIND1; HC2; HCF2; HC II; HLS2; LS2; Serpin D1; Serpin Peptidase Inhibitor Clade D Member 1; Protease inhibitor leuserpin-2 |
Assay Type: | Sandwich |
Sensitivity: | 0.71 ng/mL |
Standard: | 100 pg/mL |
Detection Range: | 1.57-100 ng/mL |
Sample Type: | plasma, |
Assay Length: | 3.5 h |
Research Area: | Hematology; |
Test Principle: | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human HCII. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human HCII. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human HCII, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human HCII in the samples is then determined by comparing the OD of the samples to the standard curve. |
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Additional information
Size | 48T, 96T, 96T,48T |
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