Overview of Rat GHRL-Ghrelin ELISA Kit
| Product name: |
Rat GHRL-Ghrelin ELISA Kit
|
| Reactivity: |
Rat |
| Alternative Names: |
MTLRP; Growth Hormone-Releasing Peptide |
| Assay Type: |
Competitive Inhibition |
| Sensitivity: |
52.3 pg/mL |
| Standard: |
10000 pg/mL |
| Detection Range: |
156.25-10000 pg/mL |
| Sample Type: |
serum, plasma, tissue homogenates, cell lysates, saliva, cell culture supernates and other biological fluids |
| Assay Length: |
2 h |
| Research Area: |
Signal transduction;Metabolic pathway;Endocrinology;Gastroenterology;Hormone metabolism; |
| Test Principle: |
This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Rat GHRL protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat GHRL. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat GHRL in the samples is then determined by comparing the OD of the samples to the standard curve. |
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