Name: Human LAP-Leucine Aminopeptidase ELISA Kit - Gencyst
SKU: ELK1958
Availability: InStock

Human LAP-Leucine Aminopeptidase ELISA Kit

€318.00 – €455.00

Specification 96T, 48T

SKU: ELK1958 Category: ELISA Kit Tag: ELISA Kit

Overview of Human LAP-Leucine Aminopeptidase ELISA Kit

Product name:	Human LAP-Leucine Aminopeptidase ELISA Kit
Reactivity:	Human
Alternative Names:	LAPEP, LAP3; PEPS; Peptidase S; Leucyl Aminopeptidase; Cytosol aminopeptidase; Proline aminopeptidase; Prolyl aminopeptidase
Assay Type:	Sandwich
Sensitivity:	0.065 ng/mL
Standard:	10 pg/mL
Detection Range:	0.16-10 ng/mL
Sample Type:	Serum, plasma, tissue homogenates and other biological fluids.
Assay Length:	3.5 h
Research Area:	Enzyme & Kinase;Metabolic pathway;Developmental science;
Test Principle:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human LAP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human LAP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human LAP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human LAP in the samples is then determined by comparing the OD of the samples to the standard curve.

Size	48T, 96T

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