Human HBF-Fetal Hemoglobin ELISA Kit
Overview of Human HBF-Fetal Hemoglobin ELISA Kit
Product name: |
Human HBF-Fetal Hemoglobin ELISA Kit |
Reactivity: | Human |
Alternative Names: | hemoglobin F; Foetal Haemoglobin |
Assay Type: | Competitive Inhibition |
Sensitivity: | 0.99 ng/mL |
Standard: | 200 pg/mL |
Detection Range: | 3.13-200 ng/mL |
Sample Type: | serum, plasma, erythrocyte lysates and other biological fluids |
Assay Length: | 2 h |
Research Area: | Hematology; |
Test Principle: | This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human HBF protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human HBF. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human HBF in the samples is then determined by comparing the OD of the samples to the standard curve. |
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Additional information
Size | 48T, 96T |
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