Human PTGS2/COX-2-Prostaglandin Endoperoxide Synthase 2 ELISA Kit
Human PTGS2/COX-2-Prostaglandin Endoperoxide Synthase 2 ELISA Kit
€318.00 – €455.00
Specification 96T, 48T
Overview of Human PTGS2/COX-2-Prostaglandin Endoperoxide Synthase 2 ELISA Kit
| Product name: | Human PTGS2/COX-2-Prostaglandin Endoperoxide Synthase 2 ELISA Kit |
| Reactivity: | Human |
| Alternative Names: | PGG/HS; PGHS2; PHS2; HCox2; COX2; Cyclooxygenase 2; Prostaglandin G/H Synthase And Cyclooxygenase; Prostaglandin H2 synthase 2; PTGS2; Prostaglandin Endoperoxide Synthase 2 |
| Assay Type: | Sandwich |
| Sensitivity: | 0.129 ng/mL |
| Standard: | 20 pg/mL |
| Detection Range: | 0.32-20 ng/mL |
| Sample Type: | serum, plasma, tissue homogenates and other biological fluids |
| Assay Length: | 3.5 h |
| Research Area: | Enzyme & Kinase;Tumor immunity;Infection immunity;Reproductive science;Pulmonology;Gastroenterology;Hormone metabolism; |
| Test Principle: | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human PTGS2/COX-2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human PTGS2/COX-2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human PTGS2/COX-2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human PTGS2/COX-2 in the samples is then determined by comparing the OD of the samples to the standard curve. |
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Additional information
| Size | 48T, 96T |
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