Human Slit2-Slit Homolog 2 ELISA Kit

Human Slit2-Slit Homolog 2 ELISA Kit

318.00455.00

Specification 96T, 48T

SKU: ELK2102 Category: Tag:

Overview of Human Slit2-Slit Homolog 2 ELISA Kit

Product name: Human Slit2-Slit Homolog 2 ELISA Kit
Reactivity: Human
Alternative Names: SLIL3
Assay Type: Sandwich
Sensitivity: 0.59 ng/mL
Standard: 100 pg/mL
Detection Range: 1.57-100 ng/mL
Sample Type: tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length: 3.5 h
Research Area: Signal transduction;Tumor immunity;Neuroscience;
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human Slit2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human Slit2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After the TMB substrate solution is added, only those wells that contain Human Slit2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a colour change. The addition of sulphuric acid solution terminates the enzyme-substrate reaction and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human Slit2 in the samples is then determined by comparing the OD of the samples to the standard curve.

Read More Description

dNTP Mix PCR Grade 10 mM each

Pfu DNA Polymerase

One Step SYBR Green RT-PCR Mix

Hot start Taq DNA Polymerase

EntiLink PCR Master Mix

Additional information

Size

48T, 96T

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