Human IL-2sRa/CD25-Soluble Interleukin-2 Receptor alpha chain ELISA Kit
Human IL-2sRa/CD25-Soluble Interleukin-2 Receptor alpha chain ELISA Kit
€318.00 – €455.00
Specification 96T, 48T
Overview of Human IL-2sRa/CD25-Soluble Interleukin-2 Receptor alpha chain ELISA Kit
Product name: |
Human IL-2sRa/CD25-Soluble Interleukin-2 Receptor alpha chain ELISA Kit |
Reactivity: | Human |
Alternative Names: | IL-2RA;CD25; IL2R-a; IL2-RA; IDDM10; IL2R; TCGFR; p55; TAC antigen; IL2Ra; Interleukin 2 Receptor Alpha |
Assay Type: | Sandwich |
Sensitivity: | 6.8 pg/mL |
Standard: | 1000 pg/mL |
Detection Range: | 15.63-1000 pg/mL |
Sample Type: | Serum, plasma, tissue homogenates and other biological fluids |
Assay Length: | 3.5 h |
Research Area: | Signal transduction;CD & Adhesion molecule;Tumor immunity;Infection immunity; |
Test Principle: | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human IL-2sRa/CD25. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human IL-2sRa/CD25. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human IL-2sRa/CD25, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human IL-2sRa/CD25 in the samples is then determined by comparing the OD of the samples to the standard curve. |
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Additional information
Size | 48T, 96T, 96T,48T |
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