Name: Human VCL-Vinculin ELISA Kit - Gencyst
SKU: ELK2218
Availability: InStock

Human VCL-Vinculin ELISA Kit

€318.00 – €455.00

Specification 96T, 48T

SKU: ELK2218 Category: ELISA Kit Tag: ELISA Kit

Overview of Human VCL-Vinculin ELISA Kit

Product name:	Human VCL-Vinculin ELISA Kit
Reactivity:	Human
Alternative Names:	MVCL; Metavinculin
Assay Type:	Sandwich
Sensitivity:	6.7 pg/mL
Standard:	1000 pg/mL
Detection Range:	15.63-1000 pg/mL
Sample Type:	serum, plasma, tissue homogenates and other biological fluids
Assay Length:	3.5 h
Research Area:	Metabolic pathway;Developmental science;Bone metabolism;
Test Principle:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human VCL. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human VCL. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human VCL, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human VCL in the samples is then determined by comparing the OD of the samples to the standard curve.

Size	48T, 96T

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