Rat OSM-Oncostatin M ELISA Kit
Rat OSM-Oncostatin M ELISA Kit
Specification 96T, 48T
Overview of Rat OSM-Oncostatin M ELISA Kit
| Product name: | Rat OSM-Oncostatin M ELISA Kit |
| Reactivity: | Rat |
| Alternative Names: | OS-M |
| Assay Type: | Sandwich |
| Sensitivity: | 6.5 pg/mL |
| Standard: | 1000 pg/mL |
| Detection Range: | 15.63-1000 pg/mL |
| Sample Type: | Serum, plasma, tissue homogenates and other biological fluids |
| Assay Length: | 3.5 h |
| Research Area: | Cytokine;Tumor immunity; |
| Test Principle: | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat OSM. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat OSM. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat OSM, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat OSM in the samples is then determined by comparing the OD of the samples to the standard curve. |
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Additional information
| Size | 96T,48T |
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