Rat MMP8-Matrix Metalloproteinase 8 ELISA Kit
Rat MMP8-Matrix Metalloproteinase 8 ELISA Kit
Specification 96T, 48T
Overview of Rat MMP8-Matrix Metalloproteinase 8 ELISA Kit
| Product name: | Rat MMP8-Matrix Metalloproteinase 8 ELISA Kit |
| Reactivity: | Rat |
| Alternative Names: | CLG2; HNC; PMNL-CL; Collagenase 2; Neutrophil Collagenase; PMNL collagenase |
| Assay Type: | Sandwich |
| Sensitivity: | 0.061 ng/mL |
| Standard: | 10 pg/mL |
| Detection Range: | 0.16-10 ng/mL |
| Sample Type: | Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids |
| Assay Length: | 3.5 h |
| Research Area: | Enzyme & Kinase;Infection immunity;Cardiovascular biology; |
| Test Principle: | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat MMP8. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat MMP8. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat MMP8, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat MMP8 in the samples is then determined by comparing the OD of the samples to the standard curve. |
Read More Description
Additional information
| Size | 96T,48T |
|---|