Rat MMP7-Matrix Metalloproteinase 7 ELISA Kit

Rat MMP7-Matrix Metalloproteinase 7 ELISA Kit

Specification 96T, 48T

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SKU: ELK2534

Overview of Rat MMP7-Matrix Metalloproteinase 7 ELISA Kit

Product name: Rat MMP7-Matrix Metalloproteinase 7 ELISA Kit
Reactivity: Rat
Alternative Names: MPSL1; MAT; PUMP-1; Matrilysin Uterine; Matrilysin; Matrin; Pump-1 Protease; Uterine Metalloproteinase
Assay Type: Sandwich
Sensitivity: 33 pg/mL
Standard: 5000 pg/mL
Detection Range: 78.13-5000 pg/mL
Sample Type: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length: 3.5 h
Research Area: Enzyme & Kinase;Tumor immunity;
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat MMP7. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat MMP7. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat MMP7, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat MMP7 in the samples is then determined by comparing the OD of the samples to the standard curve.

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Size

96T,48T

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