Rat MMP2-Matrix Metalloproteinase 2 ELISA Kit
Rat MMP2-Matrix Metalloproteinase 2 ELISA Kit
Specification 96T, 48T
Overview of Rat MMP2-Matrix Metalloproteinase 2 ELISA Kit
| Product name: | Rat MMP2-Matrix Metalloproteinase 2 ELISA Kit |
| Reactivity: | Rat |
| Alternative Names: | CLG4; CLG4A; MMP-II; MONA; TBE-1; PEX; Gelatinase A; 72kDa Gelatinase; 72kDa Type IV Collagenase |
| Assay Type: | Sandwich |
| Sensitivity: | 1.34 ng/mL |
| Standard: | 200 ng/mL |
| Detection Range: | 3.13-200 ng/mL |
| Sample Type: | serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids |
| Assay Length: | 3.5 h |
| Research Area: | Enzyme & Kinase;Tumor immunity;Infection immunity; |
| Test Principle: | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat MMP2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat MMP2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat MMP2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat MMP2 in the samples is then determined by comparing the OD of the samples to the standard curve. |
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Additional information
| Size | 96T,48T |
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