Mouse VEGFD-Vascular Endothelial Growth Factor D ELISA Kit

Mouse VEGFD-Vascular Endothelial Growth Factor D ELISA Kit

318.00455.00

Specification 96T, 48T

SKU: ELK2504 Category: Tag:

Overview of Mouse VEGFD-Vascular Endothelial Growth Factor D ELISA Kit

Product name: Mouse VEGFD-Vascular Endothelial Growth Factor D ELISA Kit
Reactivity: Mouse
Alternative Names: FIGF; VEGF-D; C-Fos Induced Growth Factor
Assay Type: Sandwich
Sensitivity: 5.7 pg/mL
Standard: 10 pg/mL
Detection Range: 15.63-1000 pg/mL
Sample Type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length: 3.5 h
Research Area: Cytokine;Tumor immunity;Bone metabolism;
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse VEGFD. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse VEGFD. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse VEGFD, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse VEGFD in the samples is then determined by comparing the OD of the samples to the standard curve.

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Size

48T, 96T, 96T,48T

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