Rat IL3-Interleukin 3 ELISA Kit
Rat IL3-Interleukin 3 ELISA Kit
Specification 96T, 48T
Overview of Rat IL3-Interleukin 5 ELISA Kit
| Product name: | Rat IL3-Interleukin 5 ELISA Kit |
| Reactivity: | Rat |
| Alternative Names: | IL-3;MCGF; MULTI-CSF; MCSF; M-CSF; Multiple; Multilineage-Colony-Stimulating Factor; Hematopoietic Growth Factor; P-Cell Stimulating Factor; Mast-Cell Growth Factor |
| Assay Type: | Sandwich |
| Sensitivity: | 2.8 pg/mL |
| Standard: | 500 pg/mL |
| Detection Range: | 7.82-500 pg/mL |
| Sample Type: | serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids |
| Assay Length: | 3.5 h |
| Research Area: | Cytokine;Infection immunity; |
| The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat IL3. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat IL3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat IL3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat IL3 in the samples is then determined by comparing the OD of the samples to the standard curve. |
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Additional information
| Size | 96T,48T |
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