Rat CTGF-Connective Tissue Growth Factor ELISA Kit
Rat CTGF-Connective Tissue Growth Factor ELISA Kit
€318.00 – €455.00
Specification 96T, 48T
Overview of Rat CTGF-Connective Tissue Growth Factor ELISA Kit
| Product name: | Rat CTGF-Connective Tissue Growth Factor ELISA Kit |
| Reactivity: | Rat |
| Alternative Names: | CCN2; HCS24; IGFBP8; NOV2; Insulin-Like Growth Factor-Binding Protein 8; Hypertrophic Chondrocyte-Specific Protein 24; CCN Family Member 2 |
| Assay Type: | Sandwich |
| Sensitivity: | 13.1 pg/mL |
| Standard: | 2000 pg/mL |
| Detection Range: | 31.25-2000 pg/mL |
| Sample Type: | serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids |
| Assay Length: | 3.5 h |
| Research Area: | Cytokine; |
| Test Principle: | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat CTGF. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat CTGF. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat CTGF, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat CTGF in the samples is then determined by comparing the OD of the samples to the standard curve. |
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Additional information
| Size | 96T,48T |
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