Overview

Human FAS(Factor Related Apoptosis) ELISA Kit

Alternative Names (Others Name):

AR; CRDGF; SDGF; Colorectum Cell-Derived Growth Factor; Schwannoma-Derived Growth Factor

 

Type of Assay:

Sandwich

Sensitivity (Percision):

5.8 pg/mL

Standard:

1000 pg/mL

 

Range:

15.63-1000 pg/mL

Type of Sample:

serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Length of Assay:

3.5h

Area of Research:

Cytokine;Tumor immunity;

Reactivity:

Mouse

Publications

 

Linearity

 

Matrix
1:2 1:4 1:8 1:16
serum(n=5) 78-92% 85-96% 93-102% 95-104%
EDTA plasma(n=5) 86-97% 91-102% 81-97% 93-107%
Heparin plasma(n=5) 82-94% 83-96% 91-103% 82-99%

 

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of AREG and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Recovery

 

Matrix Recovery range Average
serum(n=5) 93-101% 97%
EDTA plasma(n=5) 85-103% 94%
Heparin plasma(n=5) 95-105% 100%

 

Matrices listed below were spiked with certain level of recombinant AREG and the recovery rates were calculated by comparing the measured value to the expected amount of AREG in samples.

Precision

 

Intra-assay Precision (Precision within an assay):CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays):CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Standard curve

 

Concentration (ng/mL)
OD
Corrected OD
1000.00 2.154 2.046
500.00 1.647 1.539
250.00 1.157 1.049
125.00 0.726 0.618
62.50 0.514 0.406
31.25 0.318 0.210
15.63 0.216 0.108
0.00 0.108 0.000