Chicken Ntn1-Netrin 1 ELISA Kit

Chicken Ntn1-Netrin 1 ELISA Kit

367.00523.00

Specification 96T, 48T

SKU: ELK1360 Category: Tag:

Overview of Chicken Ntn1-Netrin 1 ELISA Kit

Product name: Chicken Ntn1-Netrin 1 ELISA Kit
Reactivity: Chicken
Alternative Names: NTN1L; Epididymis tissue protein Li 131P
Assay Type: Sandwich
Sensitivity: 5.4 pg/mL
Standard: 1000 ng/mL
Detection Range: 15.63-1000 pg/mL
Sample Type: Serum, plasma and other biological fluids.
Assay Length: 3.5 h
Research Area: Neuro science;
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Chicken Ntn1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Chicken Ntn1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Chicken Ntn1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Chicken Ntn1 in the samples is then determined by comparing the OD of the samples to the standard curve.

Read More Description

dNTP Mix PCR Grade 10 mM each

Pfu DNA Polymerase

One Step SYBR Green RT-PCR Mix

Hot start Taq DNA Polymerase

EntiLink PCR Master Mix

 

Additional information

Size

48T, 96T, 96T,48T

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