General AGE-Advanced Glycation End Product ELISA Kit

General AGE-Advanced Glycation End Product ELISA Kit

262.00374.00

Specification 96T, 48T

SKU: ELK1890 Category: Tag:

Overview of AGE-Advanced Glycation End Product ELISA Kit

Product name: AGE-Advanced Glycation End Product ELISA Kit
Reactivity: General
Alternative Names: AGEs; Advanced Glycation End Products
Assay Type: Competitive Inhibition
Sensitivity: 38.2 ng/mL
Standard: 8000 pg/mL
Detection Range: 125-8000 ng/mL
Sample Type: serum, plasma and other biological fluids
Assay Length: 2 h
Research Area: Metabolic pathway;Endocrinology;Cardiovascular biology;Hormone metabolism;
Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with AGE protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to AGE. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of AGE in the samples is then determined by comparing the OD of the samples to the standard curve.

Read More Description

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Additional information

Size

48T, 96T, 96T,48T

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