General BSA-Bovine Serum Albumin ELISA Kit
General BSA-Bovine Serum Albumin ELISA Kit
€262.00 – €364.00
Specification 96T, 48T
Overview of BSA-Bovine Serum Albumin ELISA Kit
| Product name: | BSA-Bovine Serum Albumin ELISA Kit |
| Reactivity: | General |
| Alternative Names: | Bovine Albumin; Fraction V |
| Assay Type: | Competitive Inhibition |
| Sensitivity: | 161.2 ng/mL |
| Standard: | 30000 pg/mL |
| Detection Range: | 468.75-30000 ng/mL |
| Sample Type: | serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids |
| Assay Length: | 2 h |
| Research Area: | Immune molecule; |
| Test principle: | This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with BSA protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to BSA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of BSA in the samples is then determined by comparing the OD of the samples to the standard curve. |
Read More Description
Additional information
| Size | 48T, 96T, 96T,48T |
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